Fig. 3.
(a) Visible spectra of adult human met-Hb (0.1 mM) in 50 mM mixed buffer (16.6 mM potassium phosphate/HEPES,/BTP) at 25 °C and upon cooling to −140 °C in presence of 50 mM potassium phosphate, TIP7, HEPES, and BTP buffers. (b) pow temperature spectra after subtraction of room temperature spectrum. All solutions measured pH 7.00 ± 0.05 at 25 °C before and after spectra were taken.