Figure 2.
The GluR2 I664C mutant shows partial inactivation under oxidizing conditions. A, Responses to 10 mm glutamate recorded in oxidizing and reducing conditions; the peak current was reduced fourfold in CuPhen but the rate of decay was similar to control conditions: control kdes 210 s–1 (blue), CuPhen kdes 220 s–1 (red); upon return to DTT, the peak amplitude was restored and the decay was faster, kdes 300 s–1 (cyan). B, Bar plot showing the increase in peak current amplitude upon switching from CuPhen to 1 mm DTT for wild-type GluR2 and the three Cys mutants; values are mean ± SEM of 5 observations per construct.