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. Author manuscript; available in PMC: 2009 Nov 10.
Published in final edited form as: Mol Ther. 2008 Sep 16;16(11):1833–1840. doi: 10.1038/mt.2008.200

Figure 3. Superior translation of Ψ-modified mrnA is independent of retinoic acid-inducible protein I (RIG-I).

Figure 3

Wild-type (wt) and RIGI(−/−) mouse embryonic fibroblasts cultured in 96-well plate (5 × 104 cells/well) were transfected with lipofectin-complexed RNA (0.25 μg/well). (a) The RNA (ppplucA50) contained unmodified uridine (U) or pseudouridine (Ψ), and had triphosphate at its 5′-end. (b) The RNA (capTEVluc-An) contained cap and long poly(A) tail. Upper panel, cells were lysed 4 hours later and luciferase activities were measured in the whole lysate. Values are measured in relative light units (RLU). Lower panel, cells were incubated for 24 hours and analyzed for interferon-β(IFN-β) by enzyme-linked immunosorbent assay. Values are expressed as the mean ± SEM (n = 4).