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. Author manuscript; available in PMC: 2010 Feb 27.
Published in final edited form as: Neuron. 2009 Aug 27;63(4):438–450. doi: 10.1016/j.neuron.2009.07.026

Figure 4. Transmission of disease from ki-3F4-FFI brain homogenates.

Figure 4

(A) Kaplan-Meier plot of Tga20 (square) and ki-3F4-WT (diamond) mice injected with 30 µl of 1% brain homogenate from 2 year old ki-3F4-WT mice. These mice never showed signs of disease.

(B–E) Kaplan-Meier plots of KO (B), WT (C), Tga20 (D), ki-3F4-WT (E), mice injected with 30 µl of 1% brain homogenates from 3 different spontaneously sick ki-3F4-FFI mice (FFI #1–3).

(F) Serial passage of ki-3F4-FFI disease from isolates (30 µl of 1% brain homogenates) derived from two separate ki-3F4-WT mice injected with FFI # 1 (E).

(G) Reactive gliosis detected by GFAP IHC was prominent in the thalamus of tga20 mice injected with brains of aged ki-3F4-FFI mice (dark staining, left) but not tga20 mice injected with brains from aged ki-3F4-WT mice (right).

(H) Enlarged ventricles (left, “V” and arrow) above the hippocampus (“H”) were also prominent in tga20 mice injected with brain homogenates from aged ki-3F4-FFI mice, but not in tga20 mice injected with brain homogenates from aged ki-3F4-WT mice (right).

(I) Reactive gliosis detected by GFAP IHC (darkly stained area) and vacuolization was prominent in the white matter of ki-3F4-WT mice injected with ki-3F4-FFI brain homogenate (left). A ki-3F4-WT mouse brain injected with brain homogenates from an aged ki-3F4-WT mouse has only background levels of GFAP staining and no vacuolization (right).

(J)Enlarged ventricle (left, arrow) above the hippocampus (Hipp) of a ki-3F4-wt mouse injected with brain homogenate from a sick ki-3F4-FFI mouse, not present in ki-3F4-WT mice injected with aged ki-3F4-WT brains (right).

(K) Enlarged ventricle above the hippocampus of a ki-3F4-WT mouse injected with brain homogenate from a sick ki-3F4-WT mouse previously injected with ki-3F4-FFI brain homogenate, not present in ki-3F4-WT mice succumbing to 22L scrapie prions (right).