Injection of artificial IMI-CCAP into LG lowers the threshold MCN1 firing frequency for activating the gastric mill rhythm in the biological preparation. A, Modest MCN1 stimulation in the absence of IMI-CCAP did not activate the gastric mill rhythm, but did elicit unitary EPSPs and action potentials in LG. Most hyperpolarized Vm: LG, −60 mV. B, A slightly faster MCN1 stimulation frequency, without IMI-CCAP injection, did elicit the gastric mill rhythm. Most hyperpolarized Vm: LG, −64 mV. C, The previously subthreshold MCN1 stimulation frequency did drive the gastric mill rhythm when artificial IMI-CCAP was injected into LG. Most hyperpolarized Vm: LG, −64 mV.