Liver homogenates were subjected to SDS-PAGE and immunoblotting for quantification of CYP2E1 protein. Top panel shows representative immunoblots from one pair of control (Con) and ethanol (EtOH)-fed mice exposed to either filtered air (FA) or ETS. Bottom panel represents the quantification of CYP2E1 protein. Data represent the mean ± SEM for n=3 pairs per group. *p<0.05, compared to corresponding control; **p<0.05, compared to ethanol + filtered air; #p<0.05, compared to control + filtered air. Two-factor ANOVA; ethanol, p<0.0001; ETS, p=0.008; ethanol × ETS, 0.17.