Figure 5. Residues involved in dimerization and Vps4 binding are required for cargo sorting in the MVB pathway.
vta1 cells (BY4742 genetic background) were transformed with both cen-copy plasmids expressing the indicated form of Vta1 and GFP-CPS. Live cells were visualized using fluorescence and brightfield microscopy and fluorescent images were processed using Deltavision deconvolution software.