Figure 3.

Analysis of astrocyte-related transcription factors. A, At E18.5, Hmgb2 has very similar expression in Olig2 knock-out (KO) spinal cords and in wild-type (Wt) littermates, whereas Tcf4 expression is ablated in Olig2 knock-out spinal cords. Tcf4 is used as a negative control. B, Klf15, an astrocyte group gene, induces ectopic GFAP expression in mouse spinal cord explant cultures. No GFAP expression is found in Olig2 or empty vector control transfected spinal cord explants. GFAP expression is also observed when the astrocyte-promoting transcription factor Scl is electroporated into the spinal cord. C, Double immunostaining showed colocalization of GFAP and Klf15 (β-gal) in P14 spinal cord of Klf15 heterozygotes (with β-gal knock-in at the Klf15 locus).