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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Mar;1789(3):204–211. doi: 10.1016/j.bbagrm.2009.01.007

Figure 2. p39 promoter sequences between −153 and +46 are sufficient for high levels of cell type-specific activity.

Figure 2

(A) Neuroblastoma N2A cells express endogenous p39. Northern blot analysis was performed with total RNA isolated from N2A cells and NIH3T3 fibroblasts. The blot was hybridized with radioactive probes specific for p39 or GAPDH transcripts; GAPDH was a control for RNA loading. (B) The p39 promoter from −217 to the ATG at +95 is sufficient for high levels of activity in neuronal N2A cells. Relative luciferase activity of reporter constructs containing 5′ truncations of the p39 promoter with the indicated end-points co-transfected with pRL-SV renilla reporter in N2A or 3T3 cells are shown. (C) p39 promoter sequences from −153 to +46 are sufficient for cell type-specific expression. Relative activities of additional 5′ and 3′ truncations with the indicated endpoints co-transfected with pRL-SV into N2A and 3T3 cells are shown. In each set of experiments, the relative luciferase activity of the longest construct in N2A cells was set at 100. The experiments were performed in triplicate; error bars indicate S.D..