Figure 3. The minimal p39 promoter sequence is highly conserved between mouse and human.
Mouse and human p39 sequences were aligned using ClustalW alignment software. Sequence identity is indicated by (*). The region between −153 and the ATG is 94% identical; sequences further upstream are not as well conserved. The five transcription start-sites identified by primer extension are indicated by forward arrows. Analysis of the minimal mouse promoter (−153/+46) (indicated by open brackets) for potential transcription factor binding sites using Transfac analysis software (http://transfac.gbf.de/TRANSFAC/) revealed 3 putative binding sites for the Sp1 family of proteins, a potential binding site for the AP-1/CREB/ATF family of proteins, and a potential E box binding site for bHLH proteins.