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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Mar;1789(3):204–211. doi: 10.1016/j.bbagrm.2009.01.007

Figure 4. Mutation of the Sp1, AP1/CREB/ATF and E box clustered binding sites reduced the activity of the p39 promoter.

Figure 4

(A) The positions of 6–10bp scanning mutations (M1–M5) in the p39 promoter region are indicated by shaded boxes. Predicted transcription factor binding sites for Sp1, AP-1/CREB/ATF and E box-binding proteins are indicated. (B) Reporter constructs containing the indicated scanning mutations in the p39 promoter construct p39-153/+46 were transfected into N2A cells and the resulting luciferase activity determined. Luciferase activity of the wild-type p39-153/+46 construct was set at 100. The experiments were performed in triplicate; error bars indicate the S.D..