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. Author manuscript; available in PMC: 2010 Mar 1.
Published in final edited form as: Biochim Biophys Acta. 2009 Mar;1789(3):204–211. doi: 10.1016/j.bbagrm.2009.01.007

Figure 7. Sp1 and Sp3 are required for activation of the p39 promoter.

Figure 7

(A) N2A cells were co-transfected with plasmid expressing shRNAs specific for either Sp1, Sp3 or control (scr). Western blot analysis with antisera specific for Sp1 (top) or Sp3 (bottom) showed transfection of the specific shRNAs reduced levels of endogenous Sp1 or Sp3 compared to the control (panel A and data not shown). Arrowheads indicate the three major Sp3 isoforms. (B) N2A cells were co-transfected with two different plasmids expressing shRNAs specific for either Sp1, Sp3 or control (scr) together with the −153/+46 or −2420/+95 p39 luciferase reporters as indicated. Luciferase activity in the scr shRNA transfected cells was set at 100. Assays were performed in triplicate; error bars indicate S.D..