Table 1. Experimental lysine-containing fragments for Efb and Efb-C.
| Chymotrypsin | ||||
|---|---|---|---|---|
| Sequencea | m/z | Unboundb | Boundc | Obsd |
| 160KQGLVK165 | 672 | N/A | N/A | Efb-C |
| 58QSRPKF63 | 762 | 1 | 1 | Efb |
| 73KHDYNIL79 | 902 | 1 | 1 | Efb |
| 64NSTPKYIKF72 | 1098 | 2 | 2 | Efb |
| 73KHDYNILEF81 | 1179 | 1 | 1 | Efb |
| 58QSRPKFNSTPKY69 | 1453 | 2 | 2 | Efb |
| 152QERIDNVLKQGLVK165 | 1640 | 1 | 1 | Efb |
| 145KVKKMVLQERIDNVL159 | 1813 | 3 | 3 | Efb-C |
| 58QSRPKFNSTPKYIKF72 | 1841 | 3 | 3 | Efb |
| 95NKPAAKTDATIKKEQKL111 | 1884 | 5 | 1 | Efb-C |
| 34GPREKKPVSINHNIVEY50 | 1980 | 1,2 | 1,2 | Efb |
| 34GPREKKPVSINHNIVEYNDGTF55 | 2514 | 1,2 | 1,2 | Efb |
| 89GARPQFNKPAAKTDATIKKEQKL111 | 2540 | 5 | 1 | Efb |
| 95NKPAAKTDATIKKEQKLIQAQNL117 | 2551 | 5 | 1 | Efb-C |
| 87EYGARPQFNKPAAKTDATIKKEQKL111 | 2833 | 5 | 1 | Efb |
| Glutamic-C | ||||
| Sequence | m/z | Unbound | Bound | Obs |
| 144YKVKKMVLQE153 | 1266 | 3 | 2 | Efb |
| 109QKLIQAQNLVRE120 | 1440/1439 | 1 | 1 | Both |
| 88YGARPQFNKPAAKTD102 | 1664 | 2 | 0 | Efb |
| 109QKLIQAQNLVREFE122 | 1716/1715 | 1 | 1 | Both |
| 32GYGPREKKPVSINHNIVE49 | 2037 | 1,2 | 1,2 | Efb |
| 88YGARPQFNKPAAKTDATIKKE108 | 2334 | 4 | 0 | Efb |
| 123KTHTVSAHRKAQKAVNLVSFE143 | 2351 | 3 | 2 | Both |
| 121FEKTHTVSAHRKAQKAVNLVSFE143 | 2627 | 3 | 2 | Both |
| 53GTFKYQSRPKFNSTPKYIKFKHDYNILEFND83 | 3826 | 4 | 4 | Efb |
a
The peptide sequence identified in peptide pool;
b
the number of acetylated lysine residues observed in unbound protein by MALDI-TOF MS;
c
the number of acetylated lysine residues observed in bound protein by MALDI-TOF MS;
d
Samples wherein the specific peptides were observed.