Figure 3.
Pdf-Gal4 drives constitutive expression of S6KII in clock cells. Top and bottom show PDF (green) and S6KII (red) immunoreactive signals, respectively, at ZT 1 and ZT 10 in pdf-Gal4; UAS-S6KII flies. S6KII could not be detected in the S6KIIign58-1 mutant, similar to results with wild-type flies (supplemental Fig. 1, available at www.jneurosci.org as supplemental material). Six brains were examined at each time point in these experiments, with similar results. See Materials and Methods for details about immunostaining procedures.