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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: Cell. 2009 May 15;137(4):635–646. doi: 10.1016/j.cell.2009.03.016

Figure 7.

Figure 7

High fat diet induced insulin resistance abolishes insulin but not metformin mediated CBP S436 phosphorylation. Mice fed regular or high fat (60% fat) diet for 4 months were treated with insulin or metformin. (A) In liver tissue from mice fed a high fat, insulin fails to stimulate CBP or Akt phosphorylation, whereas metformin maintains CBP phosphorylation capability. Each lane represents an individual mouse sample. (B) Desitometric analysis of pCBP (Ser436) and pAkt as in (A). (C) Metformin tolerance test in 16 h fasted mice (n=3) fed on high fat diet for 2 months. Means ± SEM are shown (B, C). *, =p<0.05 as compared to control mice at each time point. (D) Proposed model for the regulation of gluconeogenic gene expression by insulin and metformin.