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. Author manuscript; available in PMC: 2010 Jul 23.
Published in final edited form as: Cell. 2009 Jul 23;138(2):271–285. doi: 10.1016/j.cell.2009.05.046

Figure 1. Mobilization or inflammation induces CD47 up-regulation in hematopoietic stem and progenitor cells.

Figure 1

(A) Expression level of CD47 on c-Kit+ cells is shown for day 2 Cy/G mobilized BM. (B) Myeloid progenitor and stem cell gates are shown for day 2 mobilized BM. Histograms on right show level of CD47 expression in marrow LT-HSC and GMP for steady-state (light gray shaded histogram), day 2 mobilized (black line), and day 5 mobilized (dark gray shaded histogram). (C) Relative MFI of CD47 for GMP on days 0-5 of Cy/G mobilization. Results were normalized so that steady state GMPs had MFI 100. (D) Myeloid progenitor and stem cell gates are shown for day 2 BM post-LPS treatment. Histograms show level of CD47 expression on day 2 post-LPS (black line), day 5 post-LPS (dark gray shaded histogram), steady state (light gray shaded histogram), and IAP-/- (black shaded histogram). (E) MFI for human CD47 on HSCs from human bone marrow (NBM), cord blood CB), or mobilized peripheral blood (MPB). (F) Evaluation of KLS cells in the hematopoetic organs of IAP+/+ and IAP-/- mice mobilized on days 2 through 5. Two mice are analyzed per genotype per day.