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Pendrin abundance in the kidney can be detected by immunoblot. Pendrin band density was compared in kidney lysates from 3 wild-type and 3 pendrin null mice run in 2 separate gels. As shown, the antibody raised against the terminal 29 amino acids of the rat pendrin sequence (16) detects a protein in lysates from wild-type mice, but not from pendrin null mice, that migrates at ∼130 kDa, the expected mobility of pendrin (20). Therefore, this antibody is specific for pendrin protein.
