Fig. 4.
Effect of water intake on pendrin immunolabel. A: wild-type mice were given a balanced diet prepared as a gel and either 4 (LW) or 9 ml H2O/day (HW; series 2). After 7 days of either diet, pendrin labeling was examined in cortical sections taken from kidneys in each group. As shown, label was more distinct and discrete in the apical regions of cells in kidneys from mice given the higher water intake. B: mice were treated as described in A. Pendrin band density was quantified by immunoblotting of kidney lysates taken from mice in each group. Values were normalized to the band density obtained from kidney lysates taken from mice given the low-water diet. As shown, pendrin band density was similar in kidneys from each group.