Fig. 7.
Effect of water intake on pendrin abundance and subcellular distribution when circulating vasopressin levels are clamped. Series 4: Mice received dDAVP and 5 ml H2O/day (NW+dDAVP) or dDAVP and 11 ml H2O/day (HW+dDAVP). Pendrin labeling is shown in cortical sections from mice in each group (a). Pendrin immunolabel is shown in cortical sections from mice given 5 (A) or 11 ml H2O/day (C; series 3). CNTs from each of these micrographs are shown at higher magnification (A′ and C′). Pendrin immunolabel in CCDs from dDAVP-treated mice with the lower or higher water intake are shown in B and D. Pendrin labeling appeared more distinct and discrete in the region of the apical plasma membrane in sections from mice that consumed more H2O. b: Pendrin abundance in kidney lysates from mice in each of these treatment groups. As shown, H2O intake did not increase pendrin total protein abundance when quantified by immunoblotting (P = 0.072).