Fig. 7.
Effect of luminal (L) and/or basolateral (B) bisindolylmaleimide 1 hydrochloride (BIM;1 μM) and Gö6976 (100 nM) on flow-stimulated net cation transport in isolated perfused rabbit CCDs. A: JNa increased in untreated control (C) tubules as the luminal flow rate was increased from ∼1 to 5 nl·min−1·mm−1. BIM and Gö6976 added to the luminal and/or basolateral solutions had no effect on flow-stimulated JNa. B: JK increased ∼3-fold in response to a 5-fold increase in luminal flow rate in control CCDs. Addition of BIM and Gö6976 to the basolateral solution alone or in the presence of luminal inhibitor inhibited flow stimulation of JK. Values are means ± SE. For each protocol, n is indicated in results. *P < 0.05 compared with transport rate at 1 nl·min−1·mm−1 in same tubules. #P < 0.05 and @P = 0.05 compared with transport rate in control tubules studied at same flow rate.