Fig. 1.

Effect of trichostatin A (TSA) and AG490 on expression of α-smooth muscle actin (SMA) and fibronectin. NRK-49F cells were incubated with 100 nM TSA (A) or 40 μM AG490 (C) alone for the indicated time or together for 24 h (E) and harvested for immunoblot analysis with antibodies against α-SMA, fibronectin, or GADPH. Representative immunoblots from 3 experiments are shown. Expression levels of α-SMA or fibronectin were quantified by densitometry and are expressed as fold-induction over controls (B, D, and F). Values are means ± SD of 3 independent experiments. Bars with different letters (a–d) are significantly different from one another (P < 0.05).