Figure 5.

Bisulfite sequencing of selected genomic fragments. Bisulfite sequencing of the methylated region of the affinity precipitated M10 fragment gi|171789667: (A) 1315–1713 Contig56007; (B) the affinity precipitated M17C fragment gi|171824612: 8609–8756 Contig21050; (C) the affinity precipitated B19 fragment; (D) the selected fragment encoding for RR-2 cuticular protein located at contig124 position 21,493–22,627. The prediction of the CpG Island (shown as light gray shading) was performed by software analysis and primers were designed for bisulfite sequencing analysis using the MethPrimer program. Quantification of methylation levels was performed by direct bisulfite sequencing of 10 independent clones for A,C, and D, and 20 independent clones for B.