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Relative PMNC protein expression levels of AGER1 (A), RAGE (B), and p66shc (C) in healthy 18- to 45-yr-old subjects, subjects older than 60 yr, and CKD patients. Freshly isolated PMNCs were used for Western analysis. Density data are shown as means ± sem. Western blots are representative of five subjects per group, each performed three times. *, P < 0.05; **, P < 0.01.
