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. 2009 Nov 19;4(11):e7973. doi: 10.1371/journal.pone.0007973

Figure 2. Localization of AtEHD2 and mutant forms and co-localization with a plasma membrane marker.

Figure 2

N. benthamiana leaves transiently expressing PM-rk CD3-1007-cherry [39] and AtEHD2 forms as indicated, 48 hours after transformation, were visualized under a laser-scanning-meta confocal microscope (zeiss). A AtEHD2. B point mutations. C swapped proteins. D truncated proteins. Bars = 20 µm. Arrowheads indicate nuclear localization. E SDS-PAGE analysis of the expression of the various EHD2 forms. Proteins were transiently expressed in N.benthamiana. 48 hours after injection, total plant proteins (30 µg/lane) were extracted and subjected to 12% SDS-PAGE, transferred to a nitrocellulose membrane and probed with anti-GFP antibodies.