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Leaf disks of transiently transformed N. tabacum leaves with control (GFP) or AtEHD2 forms as indicated (48 h after transformation), were floated on a 250 mM Sorbitol solution with 2.5 µg/mL EIX (as indicated). Ethylene biosynthesis was measured after 4 hours. Error bars represent the average+SE of 4 different experiments.
