Figure 3.

P-gp expression of K562/A02 cell line and K562 cell line after treatment with different reagents for 48 hours.

Notes: A) The straps developed by western blotting. Line 1. K562 blank; line 2. K562/A02 blank; line 3. K562/A02 + 2 μM DNR; line 4. K562/A02 +2 μM DNR loaded with MNPs-Fe₃O₄ (0.1 v/v); line 5. K562/A02 +2 μM DNR in conjunction with BrTet (0.5 μM); and line 6. K562/A02 + 2 μM DNR loaded with MNPs-Fe₃O₄ (0.1 v/v), synergia with BrTet (0.5 μM). B) The relative gradation calculated by the formula above.

Abbreviations: BrTet, 5-bromotetrandrine; DNR, daunorubicin; MNPs-Fe₃O₄, magnetic nanoparticles of Fe₃O₄; P-gp, P-glycoprotein.