Figure 1.

Schematic representation of Fc fusion proteins. D1muc-Fc is a soluble form of HAVCR1 containing the IgV and two-thirds of the mucin-like domain of HAVCR1 fused to the hinge and Fc regions of human IgG1. A FLAG tag consisting of 8 amino acid residues was introduced between the HAVCR1 and hinge portions. PVR-Fc, a soluble receptor form containing the whole ectodomain of the poliovirus receptor fused to the same hinge and Fc fragment of human IgG1 was used as a negative control fusion protein. Construction and purification of D1muc-Fc and PVR-Fc was done as described [17].