Figure 2.
Effect of temperature in the alteration of HAV by soluble HAVCR1. Sedimentation analysis of HAV particles treated with soluble receptors at different temperatures. Purified HAV virions were incubated with 20 μg of D1 muc-Fc or control PVR-Fc for 30 min at 4°C or 37°C, loaded onto linear 15 to 30% sucrose gradients, and ultracentrifuged. The gradient was collected from the bottom in 20 fractions, and HAV antigen in the fractions was determined by ELISA [17]. The first 15 fractions of each gradient are shown. Data are mean results from duplicate wells; duplicate values varied by less than 10%. Poliovirus native virions and empty particles labeled with 35S-methionine were used as 160S and 80S sedimentation markers. The top and bottom of the gradients are indicated.