Figure 3.

Effect of temperature in the neutralization of HAV by soluble HAVCR1. Kinetics of neutralization of cell culture-adapted HAV by soluble HAVCR1 at different temperatures. HAV(10⁶ TCID₅₀) was incubated with 20 μg of purified D1 muc-Fc or control PVR-Fc, for different times at 4°C and 37°C. After incubation, neutralization reactions were diluted 1/100 and residual infectious HAV was titrated by an ELISA end point dilution assay in 96-well plates containing confluent monolayers of GL37 cells. Values are the log₁₀ TCID₅₀/ml of HAV determined by the Reed and Muench method [30] and the standard deviations are shown as error bars. Significant differences (P < 0.05) in viral titers at 37°C and 4°C for each time point are indicated with asterisks and their corresponding p-values.