Figure 5.

EGTA inhibits the alteration of HAV by soluble HAVCR1. Sedimentation analysis of HAV alteration by soluble HAVCR1 in the presence of ethylene glycol bis(beta-aminoethyl ether)-N, N, N', N'-tetraacetic acid (EGTA). Purified HAV virions were incubated with 5 μg of D1 muc-Fc or PVR-Fc for 2 h at 37°C with or without the addition of EGTA and separated by sedimentation in linear 15 to 30% sucrose gradients as described in Figure 2. The HAV antigen in the gradient fractions was detected by ELISA. Data are the mean OD 450 nm from duplicate wells; duplicate values varied by less than 10%. Poliovirus native virions and empty particles labeled with ³⁵S-methionine were used as 160S and 80S sedimentation markers.