Figure 6.
Effect of pH on soluble HAVCR1-mediated neutralization of HAV. HAV PI (105 TCID50) was incubated with 20 μg of purified D1 muc-Fc (hatched bars) or control PVR-Fc (closed bars) in EMEM at pH 5, 6, 7, or 8 overnight at 4°C. Residual infectious HAV was titrated by an ELISA end point dilution assay. Values are the log10 TCID50 HAV determined by the Reed and Muench method [30] and the standard deviations are shown as error bars. Significant differences (p < 0.05) in titers of virus treated with D1 muc-Fc or PVR-Fc at each pH are indicated with asterisks and their corresponding p-values.