Figure 1.

Design of gene expression analyses. Microarray comparison of HR (individual samples) with pooled LR outlined the gene expression changes associated with protection against A. salmonicida. Results obtained in naïve, unvaccinated fish indicated vaccine-independent protection and were used for the filtration of data obtained in vaccinated fish. Microarray analyses found genes with expression differences between LR and HR. The real-time qPCR analyses were conduced to verify the microarray results and to compare gene expression in the infected and uninfected fish. The numbers of differentially expressed genes are presented.