Figure 3.

Immune cell markers and genes involved in recruitment and migration of immune cells. A: Real-time qPCR versus microarray analyses. Results demonstrated concordance in direction of change between the two techniques supporting differential regulation of the gene subset. Data for qPCR are ΔΔCt ± SE of 10 HR and 10 LR versus 8 fish in the unchallenged control and data for microarray are mean log₂-ER ± SE of 6 HR fish hybridized to a pooled sample of 10 LR fish. Differences between HR and LR determined with microarrays and qPCR are shown. Different capital letters (A, B) denote a difference between challenged fish (HR and LR) while small letters (a, b) denote a difference between challenged and control fish (ANOVA, P < 0.05). LECT2 - leukocyte cell-derived chemotaxin 2, SKAP2 - Src kinase-associated phosphoprotein. B: Microarray results, examples of differentially expressed genes with significant differences between vaccinated and unvaccinated fish (t-test, n = 6 and 5, p < 0.05). Results for unvaccinated fish are not shown, data are log₂-ER ± SE.