Figure 4.

Signal transducers and regulators of gene expression. The figure shows expression changes indicative of recruitment and migration of immune cells. A. Real-time qPCR versus microarray analyses. Data for qPCR are ΔΔCt ± SE of 10 HR and 10 LR versus 8 fish in the unchallenged control and data for microarray are mean log₂-ER ± SE of 6 HR fish hybridized to a pooled sample of 10 LR fish. Differences between HR and LR determined with microarrays and qPCR are shown. Different capital letters (A, B) denote a difference between challenged fish (HR and LR) while small letters (a, b) denote a difference between challenged and control fish (ANOVA, P < 0.05). NFKB1 - nuclear factor NF-kappa-B p105 subunit, NFKBIA - NF-kappa-B inhibitor alpha, CREBBP - CREB-binding protein, YY1 - YY1 transcription factor. B. Microarray results, examples of differentially expressed genes with significant differences between vaccinated and unvaccinated fish (t-test, n = 6 and 5, p < 0.05). Results for unvaccinated fish are not shown, data are log₂-ER ± SE.