Figure 5.
Anti-bacterial effectors, markers of cellular and oxidative stress. A. Real-time qPCR versus microarray analyses. Data for qPCR are ΔΔCt ± SE of 10 HR and 10 LR versus 8 fish in the unchallenged control and data for microarray are mean log2-ER ± SE of 6 HR fish hybridized to a pooled sample of 10 LR fish. Differences between HR and LR determined with microarrays and qPCR are shown. Different capital letters (A, B) denote a difference between challenged fish (HR and LR) while small letters (a, b) denote a difference between challenged and control fish (ANOVA, P < 0.05). HO - heme oxygenase; GPX3 - plasma glutathione peroxidase; GPX2 - gastrointestinal glutathione peroxidase; ALOX5AP arachidonate 5-lipoxygenase-activating protein; CCND2 - G1/S-specific cyclin D2; Antiprot1 - alpha-1-antiproteinase-like protein. B. Microarray results, examples of differentially expressed genes with significant differences between vaccinated and unvaccinated fish (t-test, n = 6 and 5, p < 0.05). Results for unvaccinated fish are not shown, data are log2-ER ± SE.