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. Author manuscript; available in PMC: 2010 Jan 31.
Published in final edited form as: Science. 2009 Jul 31;325(5940):626–628. doi: 10.1126/science.1172926

Figure 1. Transcription through a nucleosome.

Figure 1

(a) Geometry for the dual-trap optical tweezers experiments. (b) Representative trajectories of individual transcribing polymerases with or without the nucleosome at different ionic strengths. (c), (d), and (e) Probability of arrest or termination as a function of polymerase position on the DNA template at 300, 150, and 40 mM KCl respectively. Arrest is defined as a pause that lasts longer than 20 minutes or until the tether breaks. Data for transcription of bare DNA is in solid black, and nucleosome data is in semi-translucent colors. The shaded region represents the NPS.