Figure 2. Effect of the nucleosome on transcription dynamics.

In each subplot, only traces that passed the NPS are considered. (a) Pause density with a nucleosome (dashed red line) and on bare DNA (solid black line). The pink shaded area represents the predicted pause density confidence interval at the nucleosome based on the model presented in the text. The gray shaded region is the confidence interval for pause density on bare DNA used in the model. Error bars are SEM. (b) Cumulative distributions of pause durations with (solid red line) and without (solid black line) a nucleosome present. Theoretical cumulative distributions for nucleosomal (pink dashed line) and non-nucleosomal (gray dashed line) pauses. (c) Pause-free velocities with (pink) and without (gray) a nucleosome with fits to normal distributions (solid lines). The predicted values based on the diffusive model with and without a nucleosome are shown as red and black circles, respectively.