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. Author manuscript; available in PMC: 2010 Nov 6.
Published in final edited form as: Cell Stem Cell. 2009 Nov 6;5(5):527–539. doi: 10.1016/j.stem.2009.09.014

Figure 6. FoxO3 is necessary for maintaining NSC/neural progenitor quiescence.

Figure 6

(A) Increased cell proliferation in FoxO3−/− neurospheres. Cells dissociated from secondary neurospheres from 3 month-old FoxO3+/+ or FoxO3−/− mice were plated on poly-D-lysine and incubated for 1 hour with BrdU. Cells were immunostained with antibodies to BrdU. BrdU-positive nuclei were counted. Values represent mean ± SEM from 2 independent experiments with 5 littermates for each genotype. Student's t-test, ***: p<0.001.

(B) Apoptosis in FoxO3−/− NSC compared to FoxO3+/+ NSC. Freshly isolated NSC or cells dissociated from secondary neurospheres isolated from 3 month-old FoxO3+/+ or FoxO3−/− mice were stained with antibodies to cleaved caspase 3. Cleaved caspase 3-positive cells were counted. Values represent mean ± SEM from 2 independent experiments with 5 littermates for each genotype. Student's t-test, **: p<0.01.