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. Author manuscript; available in PMC: 2010 Oct 28.
Published in final edited form as: Brain Res. 2009 Aug 31;1298:1–12. doi: 10.1016/j.brainres.2009.08.066

Fig. 3.

Fig. 3

MAPK/ERK, PI3K/Akt, PKA, and CaMKIV do not contribute to androgen-induced CREB activation in hippocampal neuron cultures. DHT-induced CREB phosphorylation was significantly affected by neither (A) inhibitors of MAPK/ERK [F (5,11) = 5.3; P = 0.010] nor (B) inhibitors of PI3K/Akt, PKA, and CaMKIV [F (7,15) = 3.4; P = 0.023]. Cultures were pretreated for 2 h with kinase inhibitors 50 μM PD98059 (PD; MEK), 10 μM U0126 (U0; MEK), 10 μM LY294002 (LY; PI3K/Akt), 1 μM H89 (PKA), 10 μM KN93 (KN; CaMKIV), or vehicle, followed by exposure to 10 nM DHT for 2 h, and then were assessed by western blot using phosphorylated (p-CREB) and total (tot-CREB) CREB antibodies (43 kDa). Percent phospho-CREB is expressed as a ratio of phosphorylated to total CREB, normalized to the vehicle-treated control condition (bottom panels). Immunoblots are from representative experiments and data show means (± SEM) of the combined experiments (n = 3). *p < 0.05 relative to the vehicle-treated control condition.