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. Author manuscript; available in PMC: 2009 Nov 21.
Published in final edited form as: Circ Res. 2008 Oct 16;103(11):1309–1318. doi: 10.1161/CIRCRESAHA.108.180042

Figure 1. Lats2 induces apoptosis in cardiac myocytes.

Figure 1

(A) Neonatal rat cardiac myocytes (NRCMs) were transduced with Ad-LacZ (control) or Ad-Lats2 at 30 MOI. Immunoblot analyses were performed using anti-Lats2 antibody.. (B) NRCMs were transduced with Ad-LacZ, Ad-Lats2 or Ad-Mst1. Cytoplasmic accumulation of mono- and oligo-nucleosomes was quantitated. Values are mean ± SEM obtained from 3 experiments. *p<0.05. (C) NRCMs were transduced with Ad-LacZ and Ad-Lats2 at 30 MOI. The result shown is representative of 3 experiments.. (D) (Left) NRCMs were transduced with Ad-LacZ and Ad-Lats2 at 30 MOI in the presence or absence of caspase inhibitor (C.I.) (100μM, ApoBlock, BD Biosciences). (Right) NRCMs were transduced with Ad-LacZ or Ad-Bcl-xL at 10 MOI. Twenty-four hours after transduction, NRCMs were transduced with Ad-LacZ or Ad-Lats2 at 10 MOI. Forty-eight hours after the second transduction, cytoplasmic accumulation of mono- and oligo-nucleosomes was quantitated. Values are mean ± SEM obtained from 3 experiments. *p<0.05. Cell lysates were subjected to immunoblots for detection of Bcl-xL and α-actin.