Figure 2. Lats2 partly mediates Mst1-induced apoptosis.
(A) NRCMs were transduced with Ad-LacZ or Ad-DN-Lats2 (DN-L2) at 30 MOI. Twenty-four hours after transduction, myocytes were transduced with Ad-Mst1 at 30MOI. (B) NRCMs were transduced with Ad-LacZ and Ad-DN-Mst1 at 30 MOI. Twenty-four hours after transduction, myocytes were transduced with Ad-Lats2 (L2) at 30 MOI. In A and B, 48 hours after the second transduction, cytoplasmic accumulation of mono- and oligo-nucleosomes was quantitated. Values are mean ± SEM obtained from 3 experiments. N.S., not significant. (C) NRCMs were transduced with Ad-LacZ, Ad-Lats2 or Ad-Mst1 with different combinations (total 60 MOI). Forty-eight hours after the transduction, cytoplasmic accumulation of mono- and oligo-nucleosomes was quantitated. Values are mean ± SEM obtained from 3 experiments. *p<0.05.
