Skip to main content
. Author manuscript; available in PMC: 2009 Nov 21.
Published in final edited form as: Circ Res. 2008 Oct 16;103(11):1309–1318. doi: 10.1161/CIRCRESAHA.108.180042

Figure 3. LATS2 inhibits cardiac hypertrophy.

Figure 3

Figure 3

(A-C) NRCMs were transduced with Ad-LacZ, Ad-Lats2 or Ad-DN-Lats2. Forty-eight hours after transduction, myocytes were stimulated with or without phenylephrine (PE, 30 μM) for 48 hours. In A, myocyte surface area was determined. The cell-surface area without PE was designated as 100%. Values are mean ± SEM obtained from 3 experiments. *p<0.05. In B, total protein content was determined. The protein content in myocytes transduced with Ad-LacZ without PE was designated as 1. Values are mean ± SEM obtained from 3 experiments. *p<0.05. In C, myocytes were stained with anti-ANF antibody. The percent of myocytes expressing ANF, as determined by typical perinuclear staining, was counted. Values are mean ± SEM obtained from 3 experiments. *p<0.05. N.S, not significant. Bar = 20 μm. (D) NRCMs were transduced with or without Ad-LacZ (60MOI), Ad-Mst1 (30MOI) + Ad-LacZ (30MOI) and Ad-Mst1 (30MOI) + Ad-DNLats2 (30MOI). Fort-eight hours after transduction, myocytes were stimulated with or without PE (30 μM) for 48 hours and then myocyte surface measured. Experimental values in myocytes transduced with Ad-LacZ without PE were designated as 1. Values are mean ± SEM obtained from 3 experiments. * p<0.05