Skip to main content
. 2009 Nov 13;85(5):544–557. doi: 10.1016/j.ajhg.2009.09.019

Figure 5.

Figure 5

Gene Expression of BEAN-EXT, TK2-EXT, and FLJ27243 in Humans

(A) RT-PCR analysis for BEAN-EXT mRNA (transcripts 1 and 2 in Figure 5) showing its brain-specific expression.

(B) RT-PCR analysis for TK2-EXT mRNA (transcript 3 in Figure 5) showing higher expression in various systemic organs than in the brain.

(C) RT-PCR of FLJ27243 mRNA in the human cerebellum. Strand-specific RT-PCR shows expression of FLJ27243, represented by “RT with AS primer,” in the brain. The “S primer” represents transcription in the orientation of BEAN, and “Random Hexamer” indicates transcripts in both directions. The specificity of this strand-specific RT-PCR is confirmed by negative amplification when reverse transcriptase is omitted [(-)].

(D) Quantitative RT-PCR on BEAN, TK2, FLJ27243, and CKLF mRNAs in controls' (n = 4) and patients' (n = 2) cerebella. The locations of RT-PCR primer and probe sets (A–J) are indicated in Figure 5 (C: controls; P: patients; the scale bar represents 1 SD) (see Table S2 for probe sequences). No consistent difference was found in the expression levels of BEAN (including BEAN-EXT; probe sets: A–G), TK2 (probe sets H and I), FLJ27243 (probe set J), or CKLF (probe sets K–L) mRNAs compared in the control versus SCA31 patient groups.