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. 2009 Nov 13;85(5):581–592. doi: 10.1016/j.ajhg.2009.09.015

Figure 4.

Figure 4

Cl− Channel Activity Associated with the Expression of p.I205T and p.L140V in HEK293 Cells

(A–C)Whole-cell current records in cells transfected with (A) eGFP, (B) WT bestrophin-1, and (C) p.I205T. Currents were evoked with 450 ms voltage steps (Vm) from −120 mV to 80 mV in increments of 20 mV. The holding Vm was −50 mV.

(D) Current density is plotted as a function of Vm for cells transfected with WT bestrophin-1 (■; n = 10), p.I205T (♦; n = 10), and WT plus p.I205T (∇; n = 7).

(E) Outward conductances measured for combinations of WT and mutant bestrophins. Conductances were measured between Vm = 60–80 mV, and the values were normalized to the mean WT conductance for each series of experiments. In the p.L140V series of experiments, the number of patched cells were as follows: WT, n = 14; p.I205T, n = 12; and WT plus p.I205T, n = 14. Results are presented as mean ± standard error of the mean. An asterisk denotes a significant difference (p < 0.05).