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. 2009 Nov 13;85(5):569–580. doi: 10.1016/j.ajhg.2009.09.016

Figure 3.

Figure 3

Immunoblotting of the Modified NAGA, Agalsidase Beta, and Agalsidase Alfa

The modified NAGA, agalsidase beta, and agalsidase alfa were separated on a Tris-glycine polyacrylamide gel and stained with Coomassie brilliant blue R, and then immunoblotting analyses with anti-NAGA and anti-GLA polyclonal antibodies were performed. The results showed that there was no immunological cross-reactivity between the modified NAGA and the recombinant GLAs, i.e., agalsidase beta and agalsidase alfa. Figure labels are as follows: Coomassie blue, staining of the gel with Coomassie brilliant blue R; Anti-NAGA, immunoblotting with anti-NAGA polyclonal antibodies; and Anti-GLA, immunoblotting with anti-GLA polyclonal antibodies.