Figure 4.

Stability of the Modified NAGA

The modified NAGA was added to buffers of the indicated pH values and incubated at 37°C for 16 hr, and then MU-α-D-galactopyranoside-degrading activity was determined (A). We examined the stability of the modified NAGA under pH 4.5 (B) and pH 7.0 (C) conditions in a buffer and in plasma (D) at 37°C by following the time course after the addition of the enzyme to the buffers and plasma. As controls, agalsidase beta and agalsidase alfa were used. Error bars represent means ± SD (n = 3). The modified NAGA is more stable than the recombinant GLAs. ^∗p < 0.01 in comparisons to both agalsidase beta and agalsidase alfa; ^∗∗p < 0.05 in comparisons to both agalsidase beta and agalsidase alfa, t test.