. 2009 Nov 13;85(5):569–580. doi: 10.1016/j.ajhg.2009.09.016

Table 2.

Purification of the Modified NAGA from Conditioned Culture Medium

	Total Protein (g)	Total Activity (mmol/h)	Specific Activity (μmol/h/mg)	Relative Purity	Yield (Percent)
Medium concentration	61	55	0.9	1.0	100
Dialyzed 50%	28	53	1.9	2.1	96
(NH₄)₂SO₄ fraction
Phenyl-Sepharose	3.3	34	10	12	63
SP-Sepharose	0.13	22	1.7 × 10²	1.9 × 10²	40
Flow-through
Q-Sepharose	0.042	21	5.0 × 10²	5.6 × 10²	38