Fig. 2.

Western blotting (WB) analysis for detecting proteinase-K-digested prion protein (PrPcore) in the brains of Ka/scrapie weight-loss-type prion (Ka/W)- and Ka/scrapie obesity-type prion (Ka/O)-affected mice. Lanes 1–3 Ka/W-affected mice, lanes 4–6 Ka/O-affected mice, lane 7 Ka/scrapie. Lanes 1 and 4: mice in the first passage; lanes 2 and 5: mice in the second passage; and lanes 3 and 6: mice in the third passage. The equivalent of 0.25 µg of brain tissue was loaded into each lane. PrPcore was detected using the monoclonal antibody (mAb) T2 [19]. The molecular markers are shown on the left (kDa)