Figure 7.

Chromatin immunoprecipitation analysis of STAT5 and Bcl6 binding in female liver and in male livers that differ in STAT5 activity content. STAT5 binding to the indicated genomic regions of Bcl6 (A and B), Socs2 (C), and Cux2 (D) were analyzed by chromatin immunoprecipitation using sets of male and female chromatin samples, prepared from individual livers of untreated adult female rats (n = 5) or adult male rats with high, medium, and low liver STAT5 activity (n = 2/group). The chromatin immunoprecipitation enrichment at each STAT5-binding region was quantified relative to input DNA. Enrichment values obtain with normal rabbit IgG (controls) are shown at the right. Data shown are mean ± sem for each group of rats.