Skip to main content
. 2009 Oct 1;23(11):1914–1926. doi: 10.1210/me.2009-0242

Figure 8.

Figure 8

STAT5-binding regions of Socs2 and Cux2 bind Bcl6 and STAT5 in vitro. Binding of Bcl6 and STAT5 to EMSA probes comprised of sequences of STAT5-binding site 224 of Socs2 or the core of STAT5-binding site 14 of Cux2 were assayed using nuclear extracts (NE, 7.5 μg/lane) prepared from HEK293T cells transfected with plasmids coding for Bcl6 (lanes 1, 2, 5, 6) or with STAT5b and GH receptor and stimulated with GH for 30 min (lanes 3, 4, 7, 8). Samples were supershifted with antibody to Bcl6 (0.4 μg/lane) (sc-368, lanes 2 and 6) or STAT5 (sc-835, Santa Cruz; lanes 4 and 8). Ab, Antibody.